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The enzymatic transgalactosylation of L-ascorbic acid was investigated to synthesize a chemically stable form of L-ascorbic acid by using commercially available β-galactosidases. Among various enzymes examined, Aspergillus oryzae β-galactosidase was found to be formed the derivative of ascorbic acid in a high yield from ascorbic acid and lactose. The reaction product was isolated by ion exchange chromatography on a Dowex 1 × 8 (Cl - form) resin and Toyopearl HW-40S gel chromatography. The product was identified as 6-O-β-_D-galactopyranosyl-_L-ascorbic acid on the basis of various experimental results, viz., UV, IR, ¹H-NMR, ^(13)C-NMR, and mass spectral data.
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